The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 28, 1989
Filed:
May. 12, 1986
Applicant:
Inventors:
Koji Ushizawa, Tokyo, JP;
Akemichi Maki, Tokyo, JP;
Toshiyuki Akimoto, Tokyo, JP;
Senkichi Nagasaki, Tokyo, JP;
Miyoshi Hirata, Tokyo, JP;
Assignee:
Daiichi Pure Chemicals Co., Ltd., Tokyo, JP;
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ;
U.S. Cl.
CPC ...
435 26 ; 435 25 ;
Abstract
A 3.alpha.-hydroxysteroid contained in a sample can be quantitatively analyzed by causing a 3.alpha.-hydroxysteroid dehydrogenase to act on the sample in the presence of nicotinamide adenine dinucleotide, causing tetrazolium or a salt thereof, an electron carrier substance, and 3-oxosteroid-.DELTA..sup.1 -dihydrogenase and/or 3-oxosteroid-.DELTA..sup.4 -dehydrogenase to act on the thus-obtained reaction product, and then quantitatively analyzing the resultant formazan.