The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 07, 1989
Filed:
Jun. 04, 1984
Robert Bauer, Bristol, IN (US);
Thomas A Magers, South Bend, IN (US);
Miles Inc., Elkhart, IN (US);
Abstract
The present invention provides a stable, convenient solid state test device for the determination of ethanol in an aqueous test sample, a method for its preparation and a method for its use. The use of carrier matrix incorporated with alcohol oxidase, a peroxidatively active substance and a suitable chromogenic indicator for the determination of ethanol in aqueous samples such as body fluid samples (e.g., serum, urine or saliva) is part of the invention. The invention provides a method of incorporating the enzyme alcohol oxidase and a peroxidatively active substance into a carrier matrix with a chromogenic indicator system capable of providing a detectable response to the presence of at least 100 mg/dL ethanol in less than about 5 minutes. The method involves either (a) the use of a specialized incorporation procedure or (b) the addition of an azide to the test device. Either method overcomes the 'false positive' problem seen when solution assay reagents are incorporated at the increased concentrations necessary to provide a test device sensitive to the at least 100 mg/dL ethanol.