The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 28, 1989

Filed:

Feb. 24, 1986
Applicant:
Inventors:

Peter Doppelfeld, Leverkusen, DE;

Gary Oosta, Elkhart, IN (US);

Alexander Riebel, Leverkusen, DE;

Karl-Wilhelm Schranz, Odenthal, DE;

Assignee:

Miles Inc., Elkhart, IN (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C12Q / ; C12Q / ;
U.S. Cl.
CPC ...
435179 ; 435 14 ; 435 25 ; 435 26 ; 435 28 ; 435177 ; 435180 ; 435182 ; 435288 ;
Abstract

Proteins such as enzymes are attached directly to a polyamide or cellulose hydrate carrier. Attachment can be carried out by impregnation of the carrier with a protein solution. The carrier can be in the form of a membrane, and the polyamide can be nylon. Resulting carrier-bound proteins find utility as biocatalyst, as test strips or as chromatography materials. In a preferred embodiment, a polyamide or cellulose hydrate membrane carrier is impregnated with an enzyme selected from peroxidase, glucose oxidase, diaphorase and glucose dehydrogenase and then dried, coated with a luminol solution and again dried. The resultant membrane can be used for glucose determination by measuring chemiluminescence produced when the membrane contacts a glucose solution.


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