The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 17, 1989
Filed:
Apr. 17, 1986
Elaine L Wilson, Newlands, ZA;
BIO-Response, Inc., Hayward, CA (US);
Abstract
Human cell lines that proliferate in the absence of serum or of any macromolecular growth factors are produced by a process comprising removing serum containing culture medium from the culture of a human cell line that requires serum or macromolecular growth factors for growth, replacing the serum-containing culture medium with culture medium deprived of serum and of any macromolecular growth factors, feeding the adherent or non-adherent cells with serum-free and growth factor-free culture medium using conventional cell culture conditions, culturing the cells until they propagate reproducibly and indefinitely in the absence of serum and of any macromolecular growth factors and a serum and growth factor independent cell line has been established. The novel cell lines can be used for the production of biologically active compounds such as proteins, especially plasminogen activators.