The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 23, 1988
Filed:
Nov. 13, 1985
Applicant:
Inventor:
Pradip Ghosh-Dastidar, Los Angeles, CA (US);
Assignee:
Beatrice Companies, Inc., Chicago, IL (US);
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C07K / ;
U.S. Cl.
CPC ...
530402 ; 530408 ; 530409 ; 530344 ; 530345 ; 530412 ; 530417 ; 530370 ;
Abstract
Disclosed are improved methods for orienting polypeptide molecules containing multiple disulfide bonds into their native conformation through (1) treating the polypeptide with (a) a denaturing agent and (b) a reducing agent capable of reductively dissociating cystine disulfide bonds; (2) concurrently reducing the concentration of the reducing agent while forming a stable intermediate by reacting a disulfide containing compound with the reduced cysteine moieties of the polypeptide and; (3) forming native cystine disulfide bonds in the presence of a mild oxidizing/reducing environment while dissociating the disulfide-group polypeptide moeities.