The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 09, 1988

Filed:

Sep. 27, 1984
Applicant:
Inventors:

Jerry L Chapman, Jr, Speedway, IN (US);

Thomas D Ingolia, Indianapolis, IN (US);

Kevin R Kaster, Indianapolis, IN (US);

Stephen W Queener, Indianapolis, IN (US);

Paul L Skatrud, Greenwood, IN (US);

Assignee:

Eli Lilly and Company, Indianapolis, IN (US);

Attorneys:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C12N / ; C12P / ;
U.S. Cl.
CPC ...
4351723 ; 435 68 ; 435 70 ; 435253 ; 435254 ; 435320 ; 435886 ; 435925 ; 435926 ; 935 27 ; 935 28 ; 935 29 ; 935 56 ; 935 68 ; 935 75 ;
Abstract

A method for transforming Cephalosporium and other lower eukaryotes is disclosed. The method involves inserting a recombinant DNA cloning vector comprising a Saccharomyces cerevisiae transcriptional and translational activating sequence positioned for expression of hygromycin phosphotransferase into a host cell and then growing the host cell under selective conditions. The vectors optionally further comprise Cephalosporium ribosomal DNA and also sequences that allow for replication and selection in E. coli and Streptomyces.


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