The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 09, 1988
Filed:
Jul. 15, 1985
Paula M Keith, West Terre Haute, IN (US);
Wendy J Cain, Terre Haute, IN (US);
International Minerals & Chemical Corp., , IN (US);
Abstract
A high density fermentation process for high yield production of bovine growth hormone by transformant E. coli is described. The process employs transformant strains of E. coli containing an expression vector coding for bovine growth hormone under the control of a bacteriophage lambda promoter-operator and a plasmid containing the .lambda.cI857 gene which codes for the temperature-sensitive repressor protein. In the initial growth period, the level of dissolved oxygen in the fermentation medium is maintained at about 20% to 60% saturation and the temperature of the medium is kept at 26.degree.-30.degree. C. Production of bovine growth hormone is then induced by raising the temperature of the medium to at least about 42.degree. C. The temperature is then reduced to about 38.degree. C. to 41.degree. C. to optimize cell growth for the remainder of the induction period, during which the level of dissolved oxygen in the medium is maintained at about 10% to 40% saturation.