The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 12, 1988
Filed:
Mar. 01, 1985
Applicant:
Inventors:
Teruhiko Beppu, Suginami-ku, Tokyo, JP;
Takeshi Uozumi, Tokyo, JP;
Katsuhiko Nishimori, Tokyo, JP;
Norio Shimizu, Hitachi, JP;
Yoshiyuki Kawaguchi, Tokyo, JP;
Noboru Yanagida, Yokohama, JP;
Assignee:
Other;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C12N / ;
U.S. Cl.
CPC ...
435253 ; 4351723 ; 435320 ; 935 44 ; 935 45 ;
Abstract
Expression plasmids containing the full cDNA sequence of calf prochymosin and capable of expressing prochymosin gene in E. coli host cells are disclosed. A method is described for the preparation of said plasmids which comprises altering the spacing between the SD and ATG sequences within the E. coli trp promoter-operator region of the parent expression plasmid, pCR 701, which is known to express prochymosin gene in the E. coli host cells. These modified expression plasmids are designed to provide high expression levels of prochymosin.