The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 28, 1988

Filed:

Sep. 10, 1986
Applicant:
Inventors:

Wu-Kuang Yeh, Greenwood, IN (US);

Joe E Dotzlaf, Greenwood, IN (US);

Assignee:

Eli Lilly and Company, Indianapolis, IN (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C12N / ; C12P / ; C12D / ;
U.S. Cl.
CPC ...
435183 ; 435 47 ; 435 49 ; 435188 ; 435189 ; 435814 ; 435815 ;
Abstract

Deacetoxycephalosporin C synthetase is provided in purified form via chromatography of crude cell-free extracts over a weak anion exchange resin followed by gel filtration and hydroxylapatite chromatography, all carried out in the presence of glycerol, a C.sub.1 -C.sub.3 alkyl monohydric alcohol, e.g., ethanol, a sulfhydryl containing reducing agent, e.g., dithiothreitol, and ascorbate. The purified enzyme which possesses both expandase and hydroxylase activities can be further purified by chromatography over a strong anion exchange resin.


Find Patent Forward Citations

Yeh, Wu-Kuang. (1988). Purified enzyme and process therefor (U.S. Patent No. 4753881). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/4753881

Data source: USPTO (United States Patent and Trademark Office) public patent records. Weekly synchronization. How IDiyas builds this profile

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