The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 21, 1988
Filed:
Dec. 17, 1985
Mary Collins, Natick, MA (US);
Joseph P Dougherty, Somerville, MA (US);
Genetics Institute, Inc., Cambridge, MA (US);
Abstract
A method for determining the presence and amount of a predetermined target nucleotide sequence in a biological sample is provided. The method employs a reagent complex which consists of a labeled probe polynucleotide having a target binding region and a second polynucleotide bound to the labeled probe in a second polynucleotide binding region thereof. The second binding region is at least partially coextensive with the target binding region. This reagent complex is contacted with a sample under conditions in which the target sequence binds to the labeled probe, displacing the second polynucleotide. Labeled probe/target hybrids are separated from intact reagent complexes and the presence and amount of these hybrids are determined.