The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 17, 1988

Filed:

May. 28, 1986
Applicant:
Inventors:

Dale B Schenk, Campbell, CA (US);

Sharon K Spratt, Sunnyvale, CA (US);

Assignee:

California Biotechnology Inc., Mountain View, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N / ; C12N / ;
U.S. Cl.
CPC ...
435-7 ; 435 14 ; 435 68 ; 435 70 ; 4351723 ; 435188 ; 435810 ; 435320 ; 530350 ; 935 10 ; 935 11 ; 935 29 ; 935 47 ;
Abstract

A fused protein for use in an enzyme immunoassay system. The protein comprises an enzymatically active .beta.-galactosidase fused, at its C terminus, to an immunologically active peptide. The protein is produced using a plasmid containing a complete .beta.-galactosidase gene fused, at its 3' end, with an oligonucleotide coding for the peptide. The fused protein is designed for use in a solid-phase enzyme immunoassay system, based on immunospecific binding of the fused protein to a solid support, or in a homogeneous enzyme immunoassay system, based on enzyme inhibition resulting from immunospecific binding of an antibody to the protein.


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