The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 10, 1988
Filed:
Feb. 24, 1986
Applicant:
Inventors:
Charles M Cohen, Medway, MA (US);
Roberto Crea, Bulingame, CA (US);
Assignee:
Creative Biomolecules, Inc., Hopkinton, MA (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C07K / ; C07K / ; C12N / ;
U.S. Cl.
CPC ...
530350 ; 530324 ; 935 47 ; 935 48 ;
Abstract
Disclosed is a process for producing a fusion protein comprising epidermal growth factor (EGF) attached through a Glu-residue to a leader. Treatment of the fusion protein with a Staphylococcus aureus V8 protease specific for cleaving peptides at a Glu-linkage produces large amounts of the 53 amino acid EGF sequence. The treatment conditions optimize production of the 53 and 51 amino acid EGFs relative to shorter analogs, and take full advantage of the selectivity of the protease.