The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 10, 1988
Filed:
Sep. 26, 1984
Applicant:
Inventors:
Eileen A Friedman, New York, NY (US);
Martin Lipkin, New York, NY (US);
Assignee:
Sloan-Kettering Institute for Cancer Research, New York, NY (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N / ; C12Q / ;
U.S. Cl.
CPC ...
43524023 ; 435 29 ; 435 30 ; 435 34 ; 435 35 ; 435244 ;
Abstract
The invention concerns a method for the routine primary culture of human colonic epithelial cells within a monolayer. 12-0-tetradecanoylphorbol-13-acetate (TPA) enhanced DNA synthesis an average of 8 fold when assayed by [.sup.3 h]dThd labeling indices (L.I.) in colonic epithelial cells from familial polyposis patients and in tubular adenomas. No such stimulation by TPA was seen in cells from high-risk patients without familial polyposis, or in cells from low-risk subjects.