The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 01, 1988

Filed:

Apr. 08, 1985
Applicant:
Inventors:

John W Roberts, Milford, MA (US);

Karen E DeBell, Easton, MA (US);

Assignee:

Cambridge BioScience Corporation, Worcester, MA (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; C12Q / ;
U.S. Cl.
CPC ...
435 34 ; 435 26 ; 435810 ;
Abstract

A tetrazolium-based system for testing for the presence of organisms or cells in a specimen includes in one embodiment a mixture of a substrate and an indicator system, wherein the overall system is buffered to a pH above 8.0, and in a preferred embodiment at about a pH of 9.0 when mixed. The substrate solution and indicator solution are not mixed until shortly before use. The indicator solution is buffered at a pH of approximately 3.3, thereby enhancing the shelf life. The substrate may consist of glucose with at least one amino acid in a buffered solution formulated such that, by mixing equal parts with the indicator solution, a pH above 8, preferably 9.0 results.


Find Patent Forward Citations

Loading…