The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 09, 1988
Filed:
Feb. 13, 1984
Applicant:
Inventors:
Randall G Rupp, Medfield, MA (US);
Scott Geyer, Allston, MA (US);
Assignee:
Damon Biotech, Inc., Needham Heights, MA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P / ; C12N / ;
U.S. Cl.
CPC ...
435 68 ; 435 70 ; 43524022 ; 43524027 ; 43524031 ;
Abstract
Disclosed is a method of improving protein production in protein-producing cell cultures. The method comprises the steps of culturing protein-producing cells in a medium comprising essential nutrients, vitamins, salts and amino acids, modified by the addition of increased amounts of amino acids so that the osmolarity of the medium is hypertonic, i.e., above about 340 milliosmoles, preferably within the range of 340 to 450 milliosmoles and most preferably 360 milliosmoles. Cells grown in such media produce protein at levels on the order of four times the protein production in normal, isotonic media.