The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 05, 1988
Filed:
Jan. 26, 1984
Applicant:
Inventor:
Thomas H Schulte, Cary, NC (US);
Assignee:
Becton, Dickinson and Company, Franklin Lakes, NJ (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ;
U.S. Cl.
CPC ...
435 30 ; 435 29 ; 435 34 ;
Abstract
A method for the detection of microorganisms e.g. bacteria in a blood sample comprises staining the microorganisms with a fluorescent dye and observing the fluorescence emission of an expanded buffy coat. The expanded buffy coat is obtained by centrifuging the stained sample in a hematocrit tube containing a float which occupies most of the buffy coat volume. The expanded buffy coat further separates into sub-layers. Intracellular microorganisms congregate in and are detected in the granulocyte sub-layer. The method may be modified to assess phagocytic activity.