The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 01, 1987
Filed:
Sep. 25, 1984
Applicant:
Inventors:
Charles L Hershberger, New Palestine, IN (US);
Jeffrey L Larson, Indianapolis, IN (US);
Assignee:
Eli Lilly and Company, Indianapolis, IN (US);
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12P / ; C12N / ; C12N / ; C12N / ; C12R / ;
U.S. Cl.
CPC ...
435 91 ; 4351721 ; 4351723 ; 435253 ; 435886 ; 435320 ; 935 31 ; 935 55 ; 935 56 ; 935 57 ; 935 58 ; 935 73 ; 935 75 ; 935 79 ; 935 80 ;
Abstract
A method of cloning endogenously modified Streptomycetes DNA, which is normally rejected by restrictionless heterospecific hosts, is disclosed. The method uses bacteriophage lambda to construct a genomic library of modified Streptomycetes DNA; such lambda-containing Streptomycetes DNA is replicated to provide a source of non-modified Streptomycetes DNA. This non-modified DNA is subcloned into a selectable cloning vector and used to transform restrictionless hetero-specific hosts. The transformants can then be screened for clones containing genes of interest.