The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 27, 1987
Filed:
Oct. 23, 1985
Morton A Vodian, Escondido, CA (US);
Eric S Bean, San Diego, CA (US);
Synbiotics, Corporation, San Diego, CA (US);
Abstract
The performance of immunoassays for the analysis of serum analytes can be significantly improved by the pretreatment of the sample. Analyte in serum samples is often complexed with serum antibody. Such analyte-antibody complexes can mask the analyte and interfere with analyte specific binding steps of many immunoassays. The serum pretreatment method employs pH dependent chaotropes to dissociate the analyte-antibody complexes in the serum. At low pH, the complexes become dissociated and the antibody becomes denatured. After the dissociation and denaturation of the serum antibody, the serum sample and the chaotrope, contained therein, are then neutralized. Since the serum antibody has been denatured, it does not re-associate with the analyte upon neutralization. After the neutralization step, serum sample can then be analyzed by an immunoassay, without interference from serum antibody. A serum pretreatment kit is essential to the employment of the serum pretreatment method.