The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 22, 1987

Filed:

Jul. 22, 1983
Applicant:
Inventors:

Kazuhiko Yamanishi, Tokyo, JP;

Toshiro Hanada, Kawagoe, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ; C12Q / ; C12Q / ; C12Q / ; C12Q / ;
U.S. Cl.
CPC ...
435 10 ; 435 11 ; 435 14 ; 435 25 ; 435 28 ; 435810 ;
Abstract

Cholesterol in a sample can be determined quantitatively by measuring colorimetrically superoxide ion generated by treating the sample with cholesterol oxidase. Similarly, a substrate such as glucose, etc., can be determined quantitatively by measuring the generated superoxide ion colorimetrically, said oxidase treatment and measuring of the generated superoxide ion being conducted by using a reagent composition comprising (a) an oxidase, (b') iron complex of porphyrin or ion chelate of complexane, (c) an amine and/or a phenol, (d) a thiol compound, (e) a color producing reagent to be reduced, and if necessary (f) a chelating agent.


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