The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 15, 1987

Filed:

Jan. 16, 1984
Applicant:
Inventors:

James D Mansour, Raleigh, NC (US);

Thomas H Schulte, Cary, NC (US);

Vernon R Neece, Durham, NC (US);

Assignee:

Becton, Dickinson and Company, Franklin Lakes, NJ (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; C12Q / ; C12Q / ; C12Q / ; G07K / ; G01N / ;
U.S. Cl.
CPC ...
435 34 ; 435 19 ; 435 29 ; 435 39 ; 435269 ; 2504612 ;
Abstract

A method for rapid detection of microorganisms in a body fluid sample includes detecting the microorganisms after treatment of the sample with a lysing agent in order to dissolve sample components other than microorganisms, and staining with a fluorescent dye. The lysing agent may be part of a composition which includes a culture medium thereby providing simultaneous lysis and growth before staining. Detection is preferably accomplished by reliance on the fluorescence emitted by the dye having been properly excited by light energy. A composition suitable for use in the above-described method includes a growth medium and a lysing agent.


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