The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 11, 1987
Filed:
Oct. 17, 1984
Applicant:
Inventor:
James F Drake, Minneapolis, MN (US);
Assignee:
Minnesota Mining and Manufacturing Company, St. Paul, MN (US);
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; C12Q / ; C12Q / ;
U.S. Cl.
CPC ...
435 24 ; 435 23 ; 435 28 ; 435 29 ; 435810 ; 435 25 ; 436 23 ;
Abstract
A method of detecting a beta-lactam antibiotic in a liquid sample comprising the steps of: (a) forming a reaction medium by adding the sample to a predetermined amount of a dry reagent formulation comprising a D,D-carboxypeptidase, a substrate for said D,D-carboxypeptidase containing a carboxy-terminal D-alanine, and a reagent system which produces a color change in the presence of D-alanine; (b) incubating said reaction medium for a predetermined period; (c) adding a quenching material to terminate the reaction; (d) observing the color of the reaction medium. A kit for carrying out this method is also disclosed.