The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 04, 1987
Filed:
Jul. 29, 1985
Applicant:
Inventors:
Robbert A Schilperoort, 2343 RP Oegstgeest, NL;
Frans A Krens, Scheveningen, NL;
George J Wullems, Warmond, NL;
Assignees:
Rijksuniversiteit Leiden, Leiden, NL;
Other;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ;
U.S. Cl.
CPC ...
4351723 ; 43524047 ; 935 56 ; 800-1 ;
Abstract
Genetic properties of higher plants are transformed by incubating protoplasts of the plants together with plasmid DNA. The incubation is effected in the presence of polyethylene glycol and calcium ions. Subsequently--during after-incubation--the calcium ion concentration in the incubation medium is gradually increased and at the same time the polyethylene glycol concentration is decreased. The resulting aggregates are separated, grown further separately, and examined for modification of their genetic properties.