The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 14, 1987
Filed:
Oct. 05, 1984
Applicant:
Inventors:
Barry R Bochner, Alameda, CA (US);
Kenneth C Olson, Burlingame, CA (US);
Rong-Chang Pai, Foster City, CA (US);
Assignee:
Genentech, Inc., South San Francisco, CA (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P / ; C12N / ; C12N / ;
U.S. Cl.
CPC ...
435 68 ; 4351723 ; 435317 ; 435259 ; 435803 ; 935 48 ;
Abstract
Periplasmic proteins are recovered from transformed gram negative bacteria by a process comprising freezing and thawing the cells. Advantages are obtained by culturing the cells in phosphate-limiting media and by killing the cells prior to separation of periplasmic proteins.