The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 17, 1987
Filed:
Nov. 09, 1983
Charles L Hershberger, New Palestine, IN (US);
Paul R Rosteck, Jr, Beech Grove, IN (US);
Eli Lilly and Company, Indianapolis, IN (US);
Abstract
The present invention is an improvement in the method for stabilizing and selecting host cells containing recombinant DNA. The method involves transforming a host cell with a recombinant DNA cloning vector which contains the .about.2.5 kb BglII cI repressor gene-containing restriction fragment of bacteriophage .lambda. and then lysogenizing the transformed host cell with a lysogenic organism which contains a marker which is lethal or conditionally lethal in the host cell but which is repressed in the transformed host cell by the repressor gene contained in the recombinant DNA cloning vector. The vector additionally contains a gene which codes for the expression of human proinsulin. The invention further comprises related recombinant DNA cloning vectors, transformed host cells and lysogenized transformed host cells.