The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 16, 1986
Filed:
Sep. 30, 1983
Erich Elstner, Grobenzell, DE;
Boehringer Mannheim GmbH, Mannheim, DE;
Abstract
Process for the selective production of the reduced oxygen species superoxide, hydrogen peroxide and hydroxyl radicals, wherein oxygen is reduced with NAD(P)H in the presence of an NAD(P)H-dependent, non-autoxidisable diaphorase, of an appropriate autoxidisable redox partner and optionally of an appropriate buffer system. Also, process for the determination of superoxide dismutase in which a superoxide-yielding reaction is coupled with two competing superoxide-consuming reactions, one superoxide-consuming reaction being a conventional indicator reaction and the other superoxide-consuming reaction being the superoxide dismutase reaction, wherein, as superoxide-yielding reaction, there is used the reduction of oxygen with NAD(P)H in the presence of an NAD(P)H-dependent, non-autoxidisable diaphorase, of an autoxidisable one-electron step-inducing redox partner with a one-electron redox potential in the range of from -150 mV to -500 mV and optionally of an appropriate buffer system. Also, process for the determination of NAD(P)H and of NAD(P)H-yielding reactions in which oxygen is reduced to hydrogen peroxide by NAD(P)H and the hydrogen peroxide is determined in knowm manner, wherein the reduction of the oxygen by NAD(P)H is carried out in the presence of an NAD(P)H-dependent, non-autoxidisable diaphorase, of an appropriate two-electron redox partner with a two-electron redox potential in the range of from 0 mV to 150 mV and of an appropriate buffer system. Also, reagents for carrying out these processes.