The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 02, 1986
Filed:
Aug. 31, 1983
Applicant:
Inventor:
Guido Grandi, Segrate, IT;
Assignee:
Eni-Ente Nazionale Idrocarburi, Roma, IT;
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N / ; C12N / ; C12P / ;
U.S. Cl.
CPC ...
435317 ; 4351723 ; 435 91 ; 435 68 ; 935 27 ; 935 74 ; 935 73 ;
Abstract
A special description is given of plasmids pSM15, pSM16 and pSM17, which are hybrid plasmids derived by coupling pUB110 and modified pE194. The described plasmids are cloning vectors in Bacillus subtilis and are characterised by a marker of resistance to Kanamycin and an EcoRI site located near the end of the ribosome recognition sequence; they can also be used to induce the coded heterologous protein from cloned DNA with sub-inhibiting doses of erythromycin. These plasmids are prepared via the formation of plasmids pSM4, pSM5 or pSM6, which can be expressed either in B. subtilis or in E. coli.