The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 14, 1986
Filed:
Sep. 19, 1984
Johnny W Peterson, Dickinson, TX (US);
Board of Regents, University of Texas System, Austin, TX (US);
Abstract
A plate of suitable nutrient medium and agar is inoculated with bacteria from a biological sample. Bacterial colonies are cultured on the plate and are then overlaid with a soft agar layer containing bacterial lysing agents. A removable sheet with bound first animal antibodies for cholera toxin is used to contact the overlaid lysed colonies. The sheet is then exposed to a second animal antibody to cholera toxin followed by treatment with a third animal antibody against the second animal antibody, said third animal antibody being coupled to an enzyme capable of generating a chromophoric product. The sheet is then developed by immersion in a substrate and system for the bound enzyme to produce chromophoric products at the sites of enterotoxigenic bacterial colonies.