The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 07, 1986
Filed:
Dec. 02, 1982
Akio Hasegawa, Shizuoka, JP;
Sadakazu Horiguchi, Shizuoka, JP;
Asahi Kasei Kogyo Kabushiki Kaisha, Osaka, JP;
Abstract
A method and medium for the multiplication of normal diploid cells from mammals is described, comprising cultivating the cells in a culture medium containing an amine derivative or derivatives represented by the general formula: NR.sup.1 R.sup.2 R.sup.3 (wherein R.sup.1, R.sup.2 and R.sup.3 represents a straight, branched or cyclic alkyl group or a hydrogen atom, provided that R.sup.1, R.sup.2 and R.sup.3 are not hydrogen atoms at the same time. Addition of these specific amine derivatives permits multiplication of normal diploid cells in a culture medium containing a limited concentration of serum. These amine derivatives are inexpensive and can be stored for a long period of time, and their storage and handling are easy. The use of these amine derivatives overcomes various problems encountered in using serum in the multiplication of normal diploid cells according to conventional methods.