The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 29, 1986
Filed:
May. 27, 1982
Applicant:
Inventor:
Donald A Kaplan, San Francisco, CA (US);
Assignee:
Other;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ; C12M / ; C12M / ;
U.S. Cl.
CPC ...
435-7 ; 435-4 ; 435 29 ; 435 34 ; 435291 ; 435297 ; 435299 ; 435810 ; 436519 ;
Abstract
Rapid screening of a large number of organisms for variants producing particular products is accomplished using membrane plates having a predetermined molecular weight cutoff and which divide a container into two chambers. High density cellular lawns are employed in conjunction with labeled antibodies for the product of interest mixed in soft agar. The cells are subjected to viral lysis and after the plaques have reached maturity, the plates are immersed in buffer. Residual soluble labeled antibody diffuses from the agar, and immunoprecipitate of the product and the antibody may then be detected.