The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 08, 1986

Filed:

Sep. 13, 1983
Applicant:
Inventors:

Paul I Terasaki, Los Angeles, CA (US);

Dennis Aoki, Los Angeles, CA (US);

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; C12M / ; C12M / ; C12M / ;
U.S. Cl.
CPC ...
435301 ; 435 33 ; 435292 ; 435294 ; 435298 ; 435299 ; 435300 ; 435293 ; 435810 ; 436180 ; 436809 ; 422 73 ; 422 99 ; 422102 ; 350536 ; 356244 ; 356246 ;
Abstract

A microdroplet test apparatus for use in HLA typing. The apparatus is an improvement upon the well known Terasaki tray which is in use for determining HLA antigens by measuring lymphocyte cytotoxicity. The apparatus includes a tray having a plurality of microtest wells wherein the sides of the microtest wells are designed to promote localization of lymphocytes or other cells being tested at the microtest well bottom. The apparatus further includes a cover having either rods or tubes which extend down into the test solution present in the microtest well to thereby control the amount of test solution which is present in the cylindrical optical view path through which the test solution is viewed by microscope for test result measurements. This allows control of the amount of test solution in the optical view path in order to reduce the amount of solution which must be looked through when cells are being counted or alternatively, increasing the amount of test solution present in the view path in order to maximize detection when a colored reaction product in the test solution is being measured.


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