The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 08, 1986
Filed:
Jul. 18, 1983
Alex M Saunders, San Carlos, CA (US);
Chin-Hai Chang, Los Altos, CA (US);
Becton, Dickinson and Company, Paramus, NJ (US);
Abstract
A method for identifying subpopulations of cells of interest without interference from other cells. In the method, a sample of at least three (3) types of cells, including a first and second type of cell which form a subpopulation of cells of interest and a third type of cell which interferes with the identification of said subpopulation, is divided into at least two (2) aliquots. A first antibody which is specific for the third type of interfering cells but not for the subpopulation, is labeled with two (2) fluorochromes, each of the fluorochromes having distinct emission spectra. The labeled first antibody is then added to a first one of the sample aliquots so as to label the third type of cells with the first antibody. A first analysis is performed by analyzing each cell to determine the fluorescence emitted and to count the third type of cells so as to distinguish the third type of cells from the subpopulation. The information obtained from the first analysis is retained for subsequent use. The above steps are repeated for the subpopulation of cells of interest and a second analysis is obtained. The retained information from the first analysis is used to remove the effect of the third type of interfering cells and to provide a direct proportional analysis of the first and second types of cells in the subpopulation of cells of interest.