The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 17, 1986
Filed:
Sep. 13, 1982
Applicant:
Inventors:
Norman D Zinder, New York, NY (US);
Peter Model, New York, NY (US);
Jef D Boeke, Cambridge, MA (US);
Assignee:
The Rockefeller University, New York, NY (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P / ; C12P / ; C12P / ; C12P / ; C12N / ; C12N / ; C12N / ; C12N / ; C12R / ;
U.S. Cl.
CPC ...
435 68 ; 435 70 ; 435 71 ; 4351723 ; 435253 ; 435261 ; 435317 ; 435849 ; 435 69 ; 935 29 ; 935 47 ; 935 48 ; 935 73 ;
Abstract
A method of externalizing proteins from the periplasmic space of gram-negative bacteria and in particular, E. coli and its relatives, comprising utilizing bacteria which have a phage gene, coding for a protein (such as gene III protein) or a mutant bacterial gene (such as a gene coding for a membrane function) which causes perturbation of the outer bacterial membrane resulting in leakage of proteins in the periplasmic space from the cell.