The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 03, 1986
Filed:
Aug. 24, 1983
Laszlo Boross, Budapest, HU;
Ivan Daroczi, Budapest, HU;
Katalin Ivony nee Kaldive, Budapest, HU;
Gabor Seres, Halasztelek, HU;
Bela Szanjani, Budapest, HU;
Jozsef Szejtli, Budapest, HU;
Reanal Finomvegyszergyar, Budapest, HU;
Abstract
The invention relates to a process for the preparation of a cyclodextrine glycosyltransferase enzyme. The immobilization can be carried out by two methods. According to the first method a cyclodextrine glycosyltransferase enzyme is activated with a solution of a carbodiimide compound and the thus treated enzyme is applied in a solution having a pH value of 4.5-8.5 onto a polysaccharide derivative comprising at least 0.1 m-equiv./g of free amino group. According to the second method the enzyme is applied in a solution having a pH value of 4.5-8.5 onto a polymer produced from an acrylic acid and/or methacrylic acid or acrylic amide and/or methacrylic amide monomer by means of a cross-linking agent of the acrylic or allylic type which polymer comprises at least 0.1 m-equiv./g of functional carboxy group, activating the polymer with a solution of a carbodiimide compound and washing and if desired drying the product thus obtained. The advantage of the process of the present invention is that the thus immobilized enzyme--contrary to the dissolved enzyme--is suitable for longlasting application and can be readily used on industrial scale continuous production.