The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 27, 1986

Filed:

Dec. 02, 1982
Applicant:
Inventors:

Lynne D Keyes, New Brunswick, NJ (US);

Douglas Testa, Neshanic Station, NJ (US);

Assignee:

Interferon Sciences, Inc., New Brunswick, NJ (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P / ; A01N / ; C12P / ; C12P / ; C12N / ; C12N / ; C01G / ;
U.S. Cl.
CPC ...
435 68 ; 435-2 ; 435 70 ; 435 91 ; 435240 ; 435241 ; 435811 ; 424101 ;
Abstract

A process and cellular system for producing soluble biological mediators, including cytokines, lymphokines, monokines and interferons, and mRNA therefor, from white blood cells is provided. The process involves treating white blood cells with a mitogenic or antigenic agent in the presence of a controlled amount of red blood cells. Increases in production of the soluble biological mediator, gamma interferon, on the order of 5 to 10 fold have been achieved by maintaining the ratio of red blood cells to white blood cells between 10 to 1 and about 50 to 1. The process is particularly useful in producing HuIFN-.gamma. with PHA-P.


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