The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 15, 1986

Filed:

May. 18, 1984
Applicant:
Inventors:

Kazuaki Oosawa, Tokyo, JP;

Nobuyoshi Ebata, Tokyo, JP;

Miyoshi Hirata, Tokyo, JP;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N / ; G01N / ;
U.S. Cl.
CPC ...
436 86 ; 436169 ; 436177 ;
Abstract

A silver staining method comprising the steps of (1) pretreating a carrier bearing thereon a substance to be detected with an alcoholic solution containing polyethylene glycol or polyoxyethylene alkylphenol, and (2) treating the pretreated carrier with a solution comprising silver nitrate, a compound of R--NH.sub.2, and a caustic alkali in ratios by molecular weight of 1:4.5-9.5:1.0-26.0. The method according to the invention involves advantages of high sensitivity and good reproducibility of detection, simple operation, safe handling etc.


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