The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 15, 1986
Filed:
Oct. 07, 1983
Pyare L Khanna, San Jose, CA (US);
Jimmy D Allen, Los Altos, CA (US);
Ian Gibbons, Menlo Park, CA (US);
Syntex (U.S.A.) Inc., Palo Alto, CA (US);
Abstract
Novel compositions and methods are provided for detecting the presence of a material of interest in a specimen on a solid surface. A composition useful for detecting the presence of a material of interest in a specimen comprises (1) a detector conjugate comprising a fluorescing moiety bonded to a compound capable of specific binding with the material of interest, or a derivative thereof, and (2) a non-detector conjugate comprising a poly(amino acid) and a compound having substantial structural and charge similarity to the fluorescing compound and no observable fluorescence, or low level fluorescence of a different wavelength than that of the fluoresing compound, in the region of fluorescence of the fluorescing compound. In the method of the invention, a specimen, on a solid surface, is combined with the detector conjugate and the non-detector conjugate, and the combination is incubated. Unbound conjugates are removed from the solid surface by washing and then the surface is irradiated with light having a wave length absorbed by the fluorescing compound. The fluorescent light emitted by the combination is observed and is a function of the amount of the material of interest present in the specimen.