The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 08, 1986

Filed:

Apr. 25, 1983
Applicant:
Inventors:

Stefan J Kirchanski, Framingham, MA (US);

Giora Davidovits, Brookline, MA (US);

Robert A Hoffman, Mansfield, MA (US);

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
G01N / ; G01N / ;
U.S. Cl.
CPC ...
435 29 ; 2504612 ; 356 39 ; 436 63 ; 436164 ; 436172 ;
Abstract

Methods for the determination of phagocytic and killing ability of selected leukocyte subclasses based upon the use of three part differentials obtained by light scatter characteristics and gated fluorescence. Particles having multiple fluorescent labels associated therewith, one of which is sensitive to reactive oxygen, incubated with the leukocytes. Detection of the fluorescent labels permits identification of cells having phagocytic ability, as determined by presence of the non-sensitive label, and also the identification of those with killing ability as determined by the decrease in fluorescence from the sensitive label.


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