The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 03, 1985
Filed:
Feb. 01, 1983
Applicant:
Inventors:
Assignee:
Agracetus, Middleton, WI (US);
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
G01N / ; G01N / ; C12Q / ; C12Q / ; C12Q / ; C12Q / ; C12Q / ;
U.S. Cl.
CPC ...
436501 ; 436504 ; 436 63 ; 436 94 ; 436518 ; 436530 ; 435-6 ; 435-5 ; 435-7 ; 435 29 ; 435 34 ; 435 39 ; 935 78 ;
Abstract
An assay method for the detection of a specific nucleotide target sequence in a polynucleotide test extract is disclosed which utilizes a polynucleotide modified probe including both a cDNA sequence substantially complementary to the specific target sequence and a protein binding sequence. The assay is conducted by exposing the modified probe to the polynucleotide test extract for hybridization and then exposing the complex to the protein which binds to the protein binding sequence. An assay, such as an immunoassay, can then be conducted on the test sample to indicate the presence of the specific target sequence by detecting the presence of the binding protein.