The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 29, 1985

Filed:

Sep. 27, 1982
Applicant:
Inventors:

Nobumoto Chikazawa, Nara, JP;

Toshiharu Muraoka, Nagaokakyo, JP;

Setsuro Fujii, Toyonaka, JP;

Assignee:

Maruho Co., Ltd., Osaka, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ; C12Q / ; C12Q / ;
U.S. Cl.
CPC ...
435 18 ; 435 25 ; 435 27 ; 435 28 ;
Abstract

The guanase activity in body fluids such as blood serum can be rapidly and accurately assayed by (I) decomposing guanine with the guanase in the specimen to xanthine and ammonia at pH 7-9, preferably at pH 8, (II) decomposing the xanthine formed by former step I with xanthine oxidase to uric acid and hydrogen peroxide, (III) reacting the reactant solution of the former step II with 3-methyl-2-benzothiazolinonehydrazone, an aniline derivative such as N,N-di-lower-alkylaniline and peroxidase, and finally measuring the optical absorption of the reactant solution of the step III at 570-600 nm. The all steps can be completed within 15 minutes. Therefore, this assay is adoptable for automatic assay of guanase on usual clinically available automatic analyzers.


Find Patent Forward Citations

Chikazawa, Nobumoto. (1985). Rapid assaying method for guanase (U.S. Patent No. 4550076). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/4550076

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