The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 21, 1985
Filed:
Jun. 01, 1984
Applicant:
Inventor:
Kenneth C Olson, Burlingame, CA (US);
Assignee:
Genentech, Inc., South San Francisco, CA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07G / ;
U.S. Cl.
CPC ...
2601 / ; 424 85 ; 435 68 ; 435 70 ; 4351723 ; 435240 ; 435241 ; 435948 ; 436548 ;
Abstract
A process for isolating and purifying a precipitated heterologous protein from a host cell culture, including treating the host cell culture with a buffered solution of ionic strength suitable to solubilize most of the host protein but in which refractile heterologous protein is substantially insoluble, and disrupting the cells to form a supernatant and an insoluble fraction; treating the insoluble fraction with a strongly denaturing solution to solubilize the refractile heterologous protein; and recovering renatured heterologous protein.