The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 05, 1985

Filed:

Dec. 07, 1981
Applicant:
Inventors:

Rolf Deeg, Seeshaupt, DE;

Helmut Schlumberger, Polling, DE;

Joachim Ziegenhorn, Starnberg, DE;

Assignee:

Boehringer Mannheim GmbH, Mannheim-Waldhof, DE;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ; C12Q / ; C12Q / ; C12Q / ; C12R / ;
U.S. Cl.
CPC ...
435 11 ; 435 19 ; 435 25 ; 435 28 ; 435805 ; 435810 ; 435886 ;
Abstract

The present invention provides a process for the determination of cholesterol in free or bound form by means of cholesterol oxidase and optionally of cholesterol esterase by measurement of the oxygen consumption, of the hydrogen peroxide formed or of the cholestenone formed, wherein the determination is carried out kinetically with the use of a cholesterol oxidase which has been obtained from a micro-organism of the genus Streptomyces and with the addition of 3,4-dichlorophenol. The present invention also provides a reagent for the kinetic determination of cholesterol, comprising cholesterol oxidase and a system for the determination of hydrogen peroxide or of cholestenone and optionally cholesterol esterase, wherein it additionally contains 3,4-dichlorophenol and the cholesterol oxidase used originates from a micro-organism of the genus Streptomyces.


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