The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 10, 1984
Filed:
Feb. 11, 1982
Douglas M Gersten, Washington, DC (US);
Edward J Zapolski, Arlington, VA (US);
Robert S Ledley, Silver Spring, MD (US);
Georgetown University, Washington, DC (US);
Abstract
Radioactive staining of gels is employed to identify proteins. A radioactive stain composition is formed by introducing radioactive iron isotope into ferrous bathophenanthroline sulfonate by adding crystalline ascorbic acid and acetic acid to a .sup.59 FeCl.sub.3 solution, adding aqueous BPS solution after the dissolution of the crystals, and adding methanol and acetic acid to yield the radioactive stain composition. Alternatively, the radioactive stain composition may be formed by bombarding a non-radioactive stain with thermal neutrons. The method of identifying proteins with the radioactive stain composition basically comprises the steps of placing the protein in the gel, forming a radioactic stain composition, and applying the radioactivated stain composition to the gel. To demonstrate the protein present, either a gamma scintillation counting technique or radioautography is preferably employed.