The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jan. 03, 1984
Filed:
May. 06, 1982
Showa Oil Company, Ltd., Tokyo, JP;
Abstract
The present invention relates to a microbiological process for the production of citric acid by assimilation of .alpha.-olefins, normal paraffins and their mixture. This process is carried out by culturing the microorganisms selected from the group of Candida tropicalis, Candida lipolytica, Candida intermedia and Canida brumptii and their mutants and their variants under higher concentration of dissolved oxygen than that in ordinary aeration in the culture medium. The concentration of dissolved oxygen in the culture medium is suitable in the range of from 5 to 40 ppm, preferably from 10 to 30 ppm on the weight basis of said culture medium in this invention. There is many means of which increase the dissolved oxygen in the culture medium. For example, in case of supplying a mixture of oxygen and air in the culture medium, the mixing ratio is suitable to from 2 parts to 25 parts of oxygen per 10 parts of air, preferably from 4 parts to 17 parts of oxygen per 10 parts of air by volume under normal temperature and pressure. It is important that the period maintaining the high concentration of dissolved oxygen in said culture medium should be immediately after the logarithmic growth phase of cultivation. Consequently, the productivity of citric acid is remarkably improved at high yield of more than 150 g/l in said fermentation process. .alpha.-olefins of C.sub.8-40, normal paraffins of C.sub.8-20 and their mixture are used as carbon source in this invention.