The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 27, 1983
Filed:
Dec. 03, 1981
David F Ranney, Dallas, TX (US);
Alfred J Quattrone, Westlake, CA (US);
Board of Regents, The University of Texas System, Austin, TX (US);
Abstract
Fluorochrome compositions and methods for the selective detection and determination of double stranded, helical DNA are provided. DNA is quantitated by determining the fluorescence enhancement imparted from the formation of a highly specific DNA-fluorochrome adduct. The fluorochrome composition disclosed includes a divalent metallic cation and a fluorescent antibiotic, antineoplastic agent selected from mithramycin, chromomycin or olivomycin. A compatible surfactant can also be included with the fluorochrome composition to enhance solubilization of cellular matter. Moreover, a compatible membrane penetrating agent such as dimethyl sulfoxide can be included with the composition to accelerate solubilization of the cellular matter. The compositions and methods provided are also useful for the screening or quantitating of agents which interact with DNA to induce displacement of fluorochrome bound to DNA.