The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 08, 1983
Filed:
Aug. 28, 1981
Applicant:
Inventors:
Jurgen Schrenk, Weilheim, DE;
Peter Wunderwald, Haunshofen, DE;
Assignee:
Boehringer Mannheim GmbH, Mannheim-Waldhof, DE;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N / ; C12R / ;
U.S. Cl.
CPC ...
435219 ; 435822 ;
Abstract
The present invention provides endoproteinase-Lys-C from bacteria, consisting of a chain of molecular weight 35000 to 38000 Dalton, having a pH optimum at pH 7.7 and being inhibited by aprotinin but not being inhibited by alpha.sub.2 -macroglobulin, .alpha..sub.1 -antitrypsin and ethylenediamine-tetraacetic acid. The present invention also provides a process for obtaining this enzyme, wherein a culture broth of an appropriate bacterial strain which has been filtered or pre-purified by usual methods is chromatographed over carrier-fixed alpha.sub.2 -macroglobulin-metal complex and the enzyme is obtained from the filtrate.