The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 19, 1983
Filed:
Dec. 15, 1981
Applicant:
Inventors:
Paul T Nix, Jackson, NJ (US);
Janet M Santoro, Freehold, NJ (US);
Joyce E Stephens, Avon-by-Sea, NJ (US);
Assignee:
Other;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q / ;
U.S. Cl.
CPC ...
435 19 ; 435198 ;
Abstract
A new stable combination of Rhizopus arrhizus lipase and Pseudomonas fluorescens lipase for hydrolyzing triglycerides to free glycerol in body fluids is disclosed. Substantially complete and rapid hydrolysis is provided by a mixture of enzymes using a ratio between 2:1 and 10:1, about 5:1 being preferred, the amounts representing units of activity of each lipase. Approximately 150 units of total lipases will provide substantially complete hydrolysis of the triglycerides contained in 20 microliters of serum up to a minimum concentration of 5 g./l. within 2.5 minutes at 37.degree. C.