The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 12, 1983
Filed:
Mar. 24, 1981
Alberto F Lopapa, Los Angeles, CA (US);
Theodore D Hall, Los Angeles, CA (US);
Lopapa Institute, Inc., Los Angeles, CA (US);
Abstract
An in-vitro diagnostic method is provided for detecting acetylsalicylic acid ingestion by a patient by utilizing radioimmunoassay procedure. The method comprises the steps of: derivatizing acetylsalicylic acid with a protein moiety being effected in a molar ratio of about one mole of protein to at least thirty moles of acetylsalicylic acid; coupling the acetylsalicylic acid derivatized protein with a water insoluble solid matrix by binding with a selected chemical agent; obtaining a sample of patient serum; freeze drying the serum including any antibodies specific to acetylsalicylic acid to concentrate the serum to a solid phase; diluting the freeze dried serum to from about 100 to about 1,000 times its original concentration; incubating the solid matrix coupled with the acetylsalicylic acid derivatized protein with the concentrated serum for a sufficient period of time to allow any antibodies specific to acetylsalicylic acid to attach to the solid matrix coupled with the acetylsalicylic acid derivatized protein, the incubation occurring for at least 24 hours at a temperature range of from about 4.degree. C. to about 42.degree. C.; thereafter, incubating the solid phase bound with specific antibodies present with radioactively labelled antibodies specific to acetylsalicylic acid derivatized protein obtained from a test animal for a sufficient period of time to allow binding between the labelled antibodies and the matrix; separating the mixture into distinct liquid and solid phases; and measuring the radiation emitted from at least one of the separated phases.