The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 07, 1983
Filed:
Jul. 13, 1981
Applicant:
Inventors:
Pasquale Sacceddu, Monterotondo, IT;
Vincenza Vitobello, Rome, IT;
Paolo Braduzzi, Rome, IT;
Nadia Cimini, Rome, IT;
Ludwig Degen, Rome, IT;
Assignee:
E.N.I. Ente Nazionale Idrocarburi, Rome, IT;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N / ; C12Q / ; C12R / ;
U.S. Cl.
CPC ...
435197 ; 435 11 ; 435824 ;
Abstract
The enzyme cholesterol esterase is produced by cultivating a strain of the Achromobacter delicatulus type, isolated from vegetable debris and identified by the symbol NRRL B-12115. Under the described culture conditions, the enzyme is exocellular and therefore does not require extraction from the cells. In addition, the micro-organism has a duplication time less than that of moulds and Actinomycetes. Cholesterol esters of fatty acids can be hydrolysed by adding them to the culture medium before inoculating the strain.