The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 09, 1982

Filed:

Dec. 23, 1980
Applicant:
Inventor:

Harvey Rubin, San Diego, CA (US);

Assignee:

Other;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C07H / ; C12N / ;
U.S. Cl.
CPC ...
536 27 ; 536 29 ; 536 30 ; 435172 ;
Abstract

A synthetic oligodeoxynucleotide complementary to endorphin mRNA and a method of using it to detect and isolate endorphin mRNA and cDNA from human and rabbit pancreas. A unique 15 base oligodeoxynucleotide dCATGAACCCGCCGTA wherein T represents thymine, G represents guanine, A represents adenine, and C represents cytosine and where at least 13 of the 15 nucleotides are as indicated (one of those indicated may be instead one of the other three mentioned nucleotides) has been found to be complementary to endorphin mRNA. To isolate endorphin mRNA, total RNA is first extracted from human brain and A+ is isolated from the total RNA. Other tissues may be used. The A+ RNA is then treated with the oligodeoxynucleotide, and the resulting hybridized RNA is enzymatically converted to endorphin mRNA:cDNA which can then be purified and used in a conventional manner to produce endorphin by cloning techniques.


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Rubin, Harvey. (1982). Detection and isolation of endorphin mrna using a synthetic (U.S. Patent No. 4358586). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/4358586

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