The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 29, 1982
Filed:
May. 28, 1980
Kevin F McGeeney, Dublin, IE;
Pharmacia Diagnostics AB, , SE;
Abstract
A method of determining the concentration of pancreatic .alpha.-amylase and salivary .alpha.-amylase in a body fluid containing a mixture of said .alpha.-amylases. First and second samples of said body fluid are prepared, and the second samples are treated with an effective amount of an inhibitor capable of selectively inhibiting the activity of said .alpha.-amylases. The amount of total .alpha.-amylase, i.e. the sum of salivary .alpha.-amylase and pancreatic .alpha.-amylase, is then measured for said first and second samples, and the concentration of salivary and pancreatic .alpha.-amylase present in said first and second samples is determined by comparing the measured total .alpha.-amylase values with a standard. The inhibitor used has a discrimination factor--expressed as the ratio between the quantity of inhibitor necessary for reducing the activity of one of said two .alpha.-amylases by 50% and the quantity of inhibitor necessary for reducing the same activity of the other of said .alpha.-amylases by 50%--of at least 20:1, preferably at least 50:1, and especially at least 80:1.